Dr. Shamoo’s research lab studies the dangerous rise of multi-drug resistant bacteria. With multi-drug resistant bacteria becoming increasingly common in hospitals, antibiotic resistance has threatened to return us to a pre-antibiotic era that would completely undermine modern medicine. His work seeks to elucidate the underlying biophysical principles of adaptation within bacterial populations during protein evolution. His group uses a combination of experimental evolution and biophysical approaches including X-ray crystallography, enzyme kinetics, protein folding, calorimetry and genomics to link changes in protein structure and function to their resulting phenotypes within evolving populations. His lab extends these physicochemical principles to predict the success or failure of specific adaptive alleles undergoing selection. By combining approaches from biophysics, genomics and experimental evolution, his group is able to identify and characterize successful evolutionary trajectories and then link those intermediates to the overall evolutionary trajectory of the bacterial populations.