Translational Technologies and Resources (Core Labs)
Director: Ponnada A. Narayana,
Contact person: Nelly Arias Nelly.Arias@uth.tmc.edu 713-500-7583
The CCTS directly supports some core laboratories (listed below) and has arranged the use of others at competitive rates at UTHealth https://www.uth.edu/research/core-labs/ and M. D. Anderson Cancer Center http://www.mdanderson.org/education-and-research/resources-for-professionals/scientific-resources/core-facilities-and-services/index.html and throughout Texas, through the Texas Regional CTSA Consortium (TRCC) http://www.utsystem.edu/offices/health-affairs/trcc-clinical-resources-and-facilities/. For more information or to use the laboratories, contact them directly or call or email Nelly Arias Nelly.Arias@uth.tmc.edu 713-500-7583.
DNA Sequencing and Genotyping Core
The core provides consultative expertise, mentoring, and technical support for studies involving genetic technologies, including DNA sequencing, genotyping techniques, data collection, and statistical analyses. The program is composed of four major components:
- Consultative and mentor-based support services that provide individual investigators with guidance in the design, application, and interpretation of their genetic studies
- Expertise in issues relating to proper informed consent for genetic studies and genetic counseling for patients enrolled in the studies.
- Sanger DNA sequencing and microsatellite and SNP genotyping services that generate preliminary data supporting translational genetic research studies.
- Infrastructure for banking DNA and other samples from patient populations and management system for sample tracking.
For more information, see the core's website https://www.uth.edu/dotAsset/654d1053-6fb9-474c-b62c-9131702cfea4.pdf
Quantitative Genomics & Microarray Core Lab
The Quantitative Genomics & Microarray Core lab provides investigators access to state-of-the-art detection technologies, automation, and expertise for a variety of scientific methodologies. The facility can perform all techniques associated with a qPCR instrument, quantitative Meso Scale ELISAs, and microarrays utilizing Illumina and Agilent arrays. The core provides a range of services that include sample preparation, instrumentation (Illumina, Light Cycler, Mesoscale, Tecan), staff consultation, and analytic software services, including Ingenuity Pathway assistance.
For more information, see the core’s website https://med.uth.edu/ibp/quantitative-genomics-microarray-facility/
Clinical and Translational Proteomics/Biomarker Core
The Clinical and Translational Proteomics Service Center provides state-of-the-art instrumentation and services to all of
- The core’s state-of-the-art instrumentation includes:
- Thermo LTQ Orbitrap XL-ETD mass spectrometer
- Agilent's 6538 Ultra High Definition Accurate-mass Q-TOF
- Agilent's 6430 Triple Quadrupole LC/ MS
- Applied Biosystems/MDS SCIEX QSTAR Elite Mass Spectrometer
- Protein identification from 1-D gel bands, 2-D spots, and other matrices
- Molecular weight determination, peptide and protein quality control
- Targeted proteomics (SRM) for biomarker discovery as well as quantitation of selected proteins or peptides in a complex mixture
- Biomarker discovery and verification
- Pharmaceutical protein quality assurance and quality control
- Characterization of immunoprecipitated or affinity-purified proteins and protein complexes
- Global protein profiling with quantitative comparisons between samples (label-free, iTRAQ, SILAC, etc)
- Enrichment and characterization of post-translationally modified proteins and peptides
For more information, see the core’s website https://www.uth.edu/imm/service-centers/clinical-translational-proteomics_copy/
Magnetic Resonance Imaging (MRI) Core
The main emphasis of the core is the development and application of advanced magnetic resonance imaging (MRI) techniques, including selective tissue imaging, magnetic resonance spectroscopic imaging, diffusion tensor imaging to neurological disorders, central nervous system trauma, and drug addiction both in humans and animal models. Another major emphasis is the development of novel image processing techniques that are robust and automatic for handling a large number of images that are typically encountered in multi-center clinical trials.
The laboratory facilities include a 7-Tesla, state-of-the-art MRI scanner dedicated to animal studies with a fully equipped animal surgical suite, a research-dedicated 3-Tesla whole-body scanner, and an image processing laboratory with a high-performance computing cluster and GPU processing.
For more information, see the core's website https://med.uth.edu/radiology/research/mr-research-group/uthealth-magnetic-resonance-imaging-core/
Atomic Force Microscopy Core
Atomic force microscopy (AFM) is a technique for characterizing surfaces and can reproduce topological images under physiological-like conditions. AFM is a noninvasive technique base on bio-sensing. Scanning can be performed in air or in a liquid environment, and a range of samples, from living cells down to single molecules, can be imaged. AFM is also an attractive tool for studying the endocytosis of
For more information, see the flyer.
Pre-Clinical Computed Tomography (CT) Core Facility
Director/Contact person: Delia Danila, PhD Delia.Danila@uth.tmc.edu 713- 486-6531
The core lab has a specialized CT system for rapid high-resolution imaging of objects up to the size of large rabbits. The facility utilizes a GE eXplore Ultra Pre-Clinical CT Scanner (GE Healthcare, London, ON) for high-resolution (150-micrometer isotropic voxel size) cone-beam CT engineered for small-animal imaging (maximum 14-cm diameter field of view). The system permits up to 1-second gantry rotation times, and X-rays are produced by a variable voltage X-ray tube capable of energies from 70 to 140 kVp. This provides high-quality images with contrast-to-noise, resolution, and
For more information, please see the following.
Bioinformatics and High-Performance Computing Service Center
The UTHealth Bioinformatics Service Center provides expertise to process and analyze biological data. Center faculty focus on gene expression, genotyping, proteomics, metabonomics, and next-generation sequencing data and can also perform custom or other complex analyses. Additional services available include experiment design and assistance in developing grant applications and manuscripts. For more information, see the Biomedical Informatics component website https://www.uth.edu/bioinformatics/
Nanochemistry Service Center
Director/Contact person: David Volk,
The Nanochemistry Service Center provides custom synthesis, labeling, and chemical coupling of DNA
For more information, see the core’s website https://www.uth.edu/imm/service-centers/nano-chemistry-service-center/
Texas Therapeutics Institute (TTI)
Director/Contact person: Zhiqiang An,
TTI is a research consortium of investigators from UTHealth, UT MDACC, and The University of Texas at Austin that promotes translational research collaborations with the pharmaceutical industry on drug discovery and drug development. TTI provides the follow-on technology and expertise to develop and optimize promising results in therapeutics research.
For more information, see the TTI website https://www.uth.edu/imm/service-centers/antibody-engineering-and-expression-service-center/