The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases

Transgenic and Stem Cell Services

Transgenic and Stem Cell

The Transgenic and Stem Cells Service Center was established in 1998 and since that time, it has generated over 750 new transgenic, knock-out and knock-in mouse animal models for investigators from UTHealth, as well as for scientists from numerous other academic institutions. The stem cell lines that have been derived in the laboratory are highly effective for the generation of knock-out/ knock-in mice and for cell differentiation studies. These cell lines are routinely used in the Core Facility and are also commercially distributed. In addition to the production, cryopreservation and re-derivation of genetically-engineered mice and rats, the services of the facility also include gene targeting, using ES cells or CRISPR/Cas9 genome editing and intellectual/technical support in different aspects of microsurgery, cell culture and stem cells research.

Services:

  • Microinjection of DNA, BAC or YAC clones for the production of transgenic mice
  • Microinjection of ES cells for the production of knock-out and knock-in mice
  • Microinjection of DNA for the production of transgenic rats
  • Gene targeting, using ES cells or CRISPR/Cas9 genome editing
  • Selection, expansion and cryopreservation of mouse ES cells
  • Cryopreservation of fertilized mouse and rat eggs and sperm
  • Re-derivation of mice and rats from fertilized eggs
  • Availability of pathogen-free animal models from high level barrier facility
  • Derivation of novel mouse ES cells and other cell lines
  • Availability of germline competent mouse ES cell lines (129/SvImJ, C57BL/6 and BALB/c) and antibiotic -resistant mouse fibroblast feeder layer cells
SUMMARY OF SERVICES FORMS (PDF forms available below).  Click here to print Transgenic Flyer

Summary of Services
Service Description

FEES
Prices Valid Through 08/31/2016

A. MICROINJECTION

UT Rates

Non UT Rates

Transgenic Mice DNA/ BAC clones (per 2 days)

$ 4,070

$ 5,600

Site – Specific integration (per 2 days)

$ 5,570

$ 7,660

CRISPR/CAS9 services (per 2 days)

$ 4,070

$ 5,600

Knock-out mice 129/SvIMJ ES cells (per day)

$ 2,090

$ 2,875

Knock-out mice C57BL/6ES cells (per day)

$ 2,420

$ 3,328

Knock-out mice BALB/c ES cells (per day)

$ 2,420

$ 3,328

 B. CRYOPRESERVATION

UT Rates

Non UT Rates

Fertilized eggs

$ 660

$ 908

Sperm (per line, minimum of 3 lines)

$ 1,100

$ 1,513

Annually Renewable Storage Fee (1st Line)

Each additional line

$ 55

 $27.50

$ 76

$38

 C. RE-DERIVATION

UT Rates

Non UT Rates

Re-Derivation of mice from embryos

$ 1,155

$ 1,589

D. ES CELL SERVICES

UT Rates

Non UT Rates

Gene targeting and selection

$ 8,250

$ 11,345

Expansion and cryopreservation

$ 715

$ 984

Derivation of MEF feeder cells

$ 2,200

$ 3,025

E. AVAILABLE CELL LINES

UT Rates

Non UT Rates

Mouse ES cells (1 vial, passage 11) 129/SVIM J

$ 660

$ 908

Mouse ES cells (1 vial, passage 11) C57/Bl/6 or BALB/c

$ 990

$ 1,362

MEF feeder cells (passage 3) 129/SvIMJ or C57BL6

$ 110

$ 152

MEF feeder cells (passage 3) DR-4

$ 110

$ 152

Contacts for further information:

Eva M. Zsigmond, Ph.D., Director
Phone: 713.500.2453 | Email: Eva.M.Zsigmond@uth.tmc.edu

Aleksey Domozhirov, Research Associate / Manager
Phone: 713.500.2452 | Email: Aleksey.Y.Domozhirov@uth.tmc.edu

UTHEALTH
The Brown Foundation Institute of Molecular Medicine
1825 Pressler Street SRB 611
Houston, Texas 77030