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Hanneman and colleagues publish biomarker validation study in Nursing Research

Hanneman is lead author of new study published in Nursing Research

(Nov. 7, 2016) – UTHealth School of Nursing’s Sandra K. Hanneman, Ph.D., R.N., FAAN, the Jerold B. Katz Distinguished Professor for Nursing Research, and professor in the Department of Acute and Continuing Care, is lead author of an article just published at that will appear in the print journal Nursing Research:

Hanneman, Sandra K.; McCue, David; Blog, Gabriel L. Validation of Salivary Interleukin-6 and Tumor Necrosis Factor-Alpha of Healthy Adult Volunteers by Enzyme Immunoassay. Nursing Research (Nov./Dec. 2016); 65(6):475-480. DOI: 10.1097/NNR.0000000000000186

Coauthors are David McCue, former manager of the Biosciences Laboratory, and alumnus Gabriel L. Blog (B.S.N., 2013), who currently is a D.N.P. student in the Nurse Anesthesia program of UTHealth School of Nursing.

The research described in the article: (1) validated the use of enzyme immunoassay developed and validated for use with blood for use with saliva for the immune biomarkers of Interleukin-6 (IL-6) and Tumor Necrosis Factor-Alpha (TNF); and (2) tested the long-term stability of immunoassay determination after 12-month storage of plasma and saliva.

Hanneman and her coauthors concluded: (1) saliva can be used to assess IL-6, but not TNF, with an immunoassay validated for use with blood, and (2) that 12-month storage of plasma and saliva significantly changes the assay results.  The results for IL-6 expand assay options for investigators and will be particularly useful for studies with longitudinal and repeated measures designs, because saliva collection is less invasive than blood collection and lends itself to multiple data collection settings, including the field and home.

The research was supported by grants from the Vivian L. Smith Foundation, PARTNERS and the Jerold B. Katz Distinguished Professorship for Nursing Research endowment.

In September, Hanneman presented the paper, “Effect of Light at Night on Salivary Melatonin Level and Circadian Rhythm” in the Pain and Symptom Management session of the prestigious biennial State of the Science Congress on Nursing Research in Washington D.C.

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