Cancer Genomics Center

Oncogenomics


The Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq)

ATAC-Seq is a rapid assay of the epigenetic state by enabling identification of open chromatin regions. In the assay, intact nuclei are treated with a hyperactive Tn5 transposase mutant which is able to simultaneously tag the target DNA with sequencing adapters and fragment the DNA in a process termed “tagmentation”. Because of the assay’s speed, simplicity, sensitivity, and applicability to a wide range of sample types, ATAC-Seq has become a commonly used epigenetic assay, and can serve as a gateway to further, more detailed, epigenetic analyses.





Sample submission requirements

Service Sample Type Minium Amount Minimum Cell Viability
ATAC-seq Cells 1 million cells per sample 80%

  • Single cells are suspended in 1xPBS
  • Samples have to be sent to core before 2 PM
  • The cells will be checked the quality and the core has the rights to determine if the samples are qualified for the experiment
  • Please check the core (cgc@uth.tmc.edu or 713-500-7933) for the detail information.


Service Workflow

Service Workflow Photo of ATAC Sequence




Useful documents