Cancer Genomics Center

The Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq)

ATAC-Seq is a rapid assay of the epigenetic state by enabling identification of open chromatin regions. In the assay, intact nuclei are treated with a hyperactive Tn5 transposase mutant which is able to simultaneously tag the target DNA with sequencing adapters and fragment the DNA in a process termed “tagmentation”. Because of the assay’s speed, simplicity, sensitivity, and applicability to a wide range of sample types, ATAC-Seq has become a commonly used epigenetic assay, and can serve as a gateway to further, more detailed, epigenetic analyses.

Sample submission requirements

Service	Sample Type	Minium Amount	Minimum Cell Viability
ATAC-seq	Cells	1 million cells per sample	80%

• Single cells are suspended in 1xPBS
• Samples have to be sent to core before 2 PM
• The cells will be checked the quality and the core has the rights to determine if the samples are qualified for the experiment
• Please check the core (cgc@uth.tmc.edu or 713-500-7933) for the detail information.

Service Workflow

Useful documents

• ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide