Ultra-low input mRNA sequencing (Ultra-low input mRNA-seq)
This sequencing service is able to generate high-quality libraries directly from live cells (100-1,000 cells) or 1-10 ng of total RNA. It provides the excellent sensitivity needed to obtain full-length transcript information from high-quality total RNA (RIN >8) or intact cells by using oligo(dT) priming. Through even gene-body coverage and accurate representation of GC-rich transcripts, this chemistry enables reliable analysis of transcript isoforms, gene fusions, point mutations, and more.
Highlights
- provide a solution for the samples with limit material
- an alternative method to supplement single-cell RNA sequencing with lower price
Sample submission requirements
Service |
Sample Type |
Recommended Amount |
Minimum Amount |
Minimum Concentration |
Quality |
Ultra-low input mRNA Seq |
Total RNA |
10 ng |
7 ng |
1 ng/ul |
Recommend: RIN>8 Required: RIN>7
|
Live Cells |
1000-100 cells |
100 cells |
|
Cell viability: >85% |
- RIN stands for RNA integrity number, which is evaluated by measuring the ratio of 28S:18S
- Concentration must be measured by Qubit
- We recommend to sort live cells at UTH flow cytometry core https://www.uth.edu/imm/service-centers/flow-cytometry/
- The core has the rights to determine if the samples are qualified for the experiment
- Please check the core ([email protected] or 713-500-7933) for the detail information.
Service Workflow

Useful documents