Total RNA sequencing (Total RNA-seq)
Total RNA sequencing service is able to profile both coding and long non-coding transcripts (>100bp). Long non-coding RNAs (lncRNAs) are a moderately abundant fraction of the eukaryotic transcriptome, including lincRNAs (intergenic lncRNAs), intronic, anti-sense, sense and bidirectional lncRNAs. Effects of lncRNAs show evidence on multiple cellular functions and perform as prime targets on the regulation of gene transcription, post-transcriptional modifications, and epigenetics. In total RNA-seq pipeline, mRNAs and lncRNAs were enriched by depleting rRNA from the samples as the figure below.

Sample submission requirements
Service |
Sample Type |
Recommend Amount |
Minium Amount |
Minium concentration |
Quality |
Total RNA-seq |
Total RNA |
1 ug |
200 ng |
40 ng/ul |
Recommend: RIN>7
Required: RIN>2.3 DV200 >66%
|
- RIN stands for RNA integrity number, which is evaluated by measuring the ratio of 28S:18S
- DV200 evaluates the percentage of fragments >200 nucleotides
- Total RNA-seq may not be available for some species
- The Core has the rights to determine whether the samples are qualified for the experiment
- Please check our Core ([email protected] or 713-500-7933) for more information.
Service Workflow

Useful documents