Total RNA sequencing (Total RNA-seq)
Total RNA sequencing service is able to profile both coding and long non-coding transcripts (>100bp). Long non-coding RNAs (lncRNAs) are a moderately abundant fraction of the eukaryotic transcriptome, including lincRNAs (intergentic lncRNAs), intronic, anti-sense, sense and bidirectional lncRNAs. Effects of lncRNAs show evidence on multiple cellular functions and perform as prime targets on the regulation of gene transcription, post-transcriptional modifications, and epigenetics. In total RNA-seq pipeline, mRNAs and lncRNAs were enriched by depleting rRNA from the samples as the figure below.
Sample submission requirements
|Service||Sample Type||Recommend Amount||Minium Amount||Minium concentration||Quality|
|Total RNA-seq||Total RNA||1 ug||200 ng||40 ng/ul||Recommend: RIN>7