mRNA sequencing service
Service workflow
Analysis pipeline (general purpose)
The Cancer Genomics Center of UTHealth can help you study transcriptomes of your samples via RNA-seq. The RNA you provided will be transferred to cDNA followed by ligation of adapters and then the cDNA will be sequenced by Illumina equipment. The major applications for RNA-seq include, but are not limited to, detecting differentially expressed genes, splicing, mutations at the mRNA level, gene fusions, and non-coding RNA expression.
Sample guideline| Service | Sample type | Minimum amount | Minimum concentration | Quality |
|---|---|---|---|---|
| mRNA-seq | Total RNA | 2 µg | 200 ng/µL | RIN>8.0 |
| mRNA-seq | mRNA | 100 ng | 5 ng/µL | RIN>8.0 |
*RIN stands for RNA integrity number, which is evaluated using the 28S to 18S rRNA ratio.
*Please use Qubit to measure DNA/RNA concentration.
*Please provide Bioanalyzer results for your sample if it is available.
An Introduction to RNA-seq
Here's a gentle introduction to the mRNA-Seq subject that covers the basics behind the experiment, how the data is processed and the sorts of things you can do with it.
Analysis pipeline (general purpose)
