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mRNA sequencing service
The Cancer Genomics Center of UTHealth can help you study transcriptomes of your samples via RNA-seq. The RNA you provided will be transferred to cDNA followed by ligation of adapters and then the cDNA will be sequenced by Illumina equipment. The major applications for RNA-seq include, but are not limited to, detecting differentially expressed genes, splicing, mutations at the mRNA level, gene fusions, and non-coding RNA expression.
|Service||Sample type||Minimum amount||Minimum concentration||Quality|
|mRNA-seq||Total RNA||1 µg||20 ng/µL||RIN>7.0|
*RIN stands for RNA integrity number, which is evaluated using the 28S to 18S rRNA ratio.
*Please use Qubit to measure DNA/RNA concentration.
*Please provide Bioanalyzer results for your sample if it is available.
Here's a gentle introduction to the mRNA-Seq subject that covers the basics behind the experiment, how the data is processed and the sorts of things you can do with it.