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Flex scRNAseq (single-cell RNAseq using fixed cells)
Single-cell RNA sequencing is becoming increasingly utilized for profiling larger sample numbers, such as cohorts of patients or diverse perturbations. Establishing an efficient and scalable workflow is crucial in this context. Paraformaldehyde (PFA) fixation enables the collection, shipment, and analysis of samples to a central location without compromising integrity or data quality. This opens up new possibilities for sample accessibility, throughput, and batched analysis. Furthermore, advanced chemistry extends single-cell transcriptomic profiling to formalin-fixed, paraffin-embedded (FFPE) tissue, broadening the range of accessible sample types. With a scalable workflow, it is now possible to profile up to a million fixed single cells simultaneously, allowing for flexibility in scheduling while meeting the specific needs of translational and clinical labs dealing with fragile samples or time constraints that would otherwise impede analysis.
Sample submission requirements
|Single cells or
Fixed RNA Profiling with Cell Ranger multi
Cell Ranger v7.0 and later supports analyzing Fixed RNA Profiling (FRP) data with the cellranger multi pipeline (see library compatibility table). FRP data cannot be analyzed with the cellranger count pipeline. Please follow the instructions of the link.