Ultra-low input mRNA sequencing (Ultra-low input mRNA-seq)
This sequencing service is able to generate high-quality libraries directly from live cells (100-1,000 cells) or 1-10 ng of total RNA. It provides the excellent sensitivity needed to obtain full-length transcript information from high-quality total RNA (RIN >8) or intact cells by using oligo(dT) priming. Through even gene-body coverage and accurate representation of GC-rich transcripts, this chemistry enables reliable analysis of transcript isoforms, gene fusions, point mutations, and more.
Highlights
- Provide a solution for the samples with limit material
- An alternative method to supplement single-cell RNA sequencing with lower price
Sample submission requirements
| Service | Sample Type | Recommended Amount | Minimum Amount | Minimum concentration | Quality |
| Ultra-low input mRNA Seq | Total RNA | 10 ng | 7 ng | 1 ng/ul | Recommend: RIN>8 Required: RIN>7 |
| Live Cells | 1000-100 cells | 100 cells | Cell viability: >85% |
- RIN stands for RNA integrity number, which is evaluated by measuring the ratio of 28S:18S
- Concentration must be measured by Qubit
- We recommend to sort live cells at UTHH Flow Cytometry Core
- The core has the rights to determine if the samples are qualified for the experiment
- Please contact the core via email at [email protected] or call 713-500-7933 for more information.
Service workflow
