The UTHealth Houston Cancer Genomics Center (CGC), a nonprofit core, was established in 2019 and funded by a CPRIT grant (RP180734). The CGC’s mission is to bring the newest next generation sequencing (NGS) technologies to the research community and to help facilitate cutting-edge cancer research in the Texas Medical Center and other areas of Texas. The CGC offers the bulk DNA sequencings, the bulk RNA sequencings and single-cell RNA sequencings. The CGC also provides project consultation and advanced bioinformatics services and collaboration.
Due to the reliable and fast-turnaround services, the core has built a user group with more than 100 investigators from UTHealth Houston, MD Anderson Cancer Center, Baylor College of Medicine, Rice University, The Methodist Hospital Research Institute, Texas Children Hospital, Texas A&M University and more. In addition, CGC service has contributed to many publications and grant applications. Through the COVID-19 pandemic, CGC has maintained reliable services and developed multiple new pipelines, including single-cell multiome (ATAC sequencing and transcriptome sequencing), Visium Spatical transcriptomcis, and ultra-low input mRNA sequencing. The new services will continue to be explored and added to support the research needs of the community.
Services
NGS services
- Free consultation on study design and sample submission
- DNA/RNA preparation, library preparation, and NGS
- Sequencing services including mRNA-seq, scRNA-seq, ChIP-seq, sRNA-seq, DNA-seq
- For other sequencing services, please contact us.
Other services
- Grant proposal development
- NGS training in bioinformatics
Bioinformatics services
- NGS data analysis (WGS, WES, microRNA, ChIP-seq, single cell RNA-seq, methylation, etc.)
- Array data analysis such as GWAS, CNV, and methylation analysis
- Advanced bioinformatics services including:
- Gene set enrichment analysis (Gene Ontology, pathways, etc.)
- Network analysis
- Disease association analysis
- Functional genomics analysis
- Results interpretation
- Machine learning and applications
- Databases
- Pharmacogenetics and pharmacogenomics data analysis
Equipment
- Illumina NextSeq 550
- 10x Genomics Chromium X Controller
- 10x Genomics Visium CytAssist
- 10x Genomics Xenium Analyzer
- Cell Countess II
- Qubit 4 DNA/RNA/Protein quantitation system
- Bio-Rad C1000 Touch Thermal Cycler with Deep-Well block
- Eppendorf Centrifuge 5430R and 5810R
- CryoCube® F740hi -80C Freezer
- QS3 QPCR System
Core personnel
- Zhongming Zhao, PhD, Director
- Wenbo Li, PhD, NGS Sequencing Leader
- Wankun Deng, PhD, Bioinformatics Leader
- Xian Chen, PhD, Program Manager
- Yulin Dai, PhD, Bioinformatics
- Citu Citu, PhD, Bioinformatics
- Zachary M. Robinson, NGS Support
- Shipra Pant, PhD, NGS Support
Acknowledgement
The CGC is supported by CPRIT Core Facility Support Grants (#RP240610). Please use one of the following formats to acknowledge in your manuscripts:
- The sequencing data was generated by the UTHealth Houston Cancer Genomics Core, supported by the Cancer Prevention and Research Institute of Texas (CPRIT RP240610).
- We thank the technical support provided by the Cancer Prevention and Research Institute of Texas (CPRIT RP240610).