Flex scRNAseq (single-cell RNAseq using fixed cells)
Single-cell RNA sequencing is increasingly being applied to large and diverse sample cohorts, including patient populations and experimental perturbation studies. Efficient, scalable workflows are therefore essential for managing sample volume while maintaining data quality and consistency. Paraformaldehyde (PFA) fixation enables samples to be collected, transported, and processed at a centralized facility while preserving sample integrity. This approach expands opportunities for distributed sample collection, increased throughput, and efficient batch processing, making it particularly suitable for longitudinal and multi-site studies.
Flex V2 supports high-throughput single-cell RNA sequencing through scalable 96-well plate-based multiplexing, with the flexibility to process either partial or full plates. Advanced probe-based chemistry and automated cell partitioning provide high cell recovery, sensitivity, and sequencing efficiency across fresh, frozen, and fixed samples, including FFPE specimens. Rapid fixation further facilitates convenient sample storage and centralized processing. Multiomic applications, including protein capture and large-scale CRISPR screening, provide additional opportunities for comprehensive cellular profiling.
Highlights
- Preserve biological states and fragile cells by fixing fresh samples at the collection point.
- Use quick and easy fixation and storage for longitudinal timepoints, multi-site studies, and more
- Detect more genes with less sequencing from fresh, frozen, and fixed samples (even FFPE and fixed whole blood)
- Achieve large-scale and highly sensitive measurements of gene expression.
Sample submission requirements
| Service | Sample Type | Minimum Amount | Morphology | Debris |
| Flex scRNAseq | Single cells or Single nuclei | 500,000 | Intact membrane | Low |
- Single cells/single nuclei are suspended in frozen media and sent on dry ice
- The cells/nuclei will be checked the quality and the core has the rights to determine if the samples are qualified for the experiment
- Please check the core ([email protected] or 713-500-7933) for the detail information.
Service workflow
Data analysis with Cell Ranger multi
Cell Ranger v10.0 and later supports analyzing Flex v2 data with the cellranger-multi pipeline. Please follow the instructions of the link.