Flex scRNA Sequencing

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Cancer Genomics Center

Flex scRNAseq (single-cell RNAseq using fixed cells)

Single-cell RNA sequencing is becoming increasingly utilized for profiling larger sample numbers, such as cohorts of patients or diverse perturbations. Establishing an efficient and scalable workflow is crucial in this context. Paraformaldehyde (PFA) fixation enables the collection, shipment, and analysis of samples to a central location without compromising integrity or data quality. This opens up new possibilities for sample accessibility, throughput, and batched analysis. Furthermore, advanced chemistry extends single-cell transcriptomic profiling to formalin-fixed, paraffin-embedded (FFPE) tissue, broadening the range of accessible sample types. With a scalable workflow, it is now possible to profile up to a million fixed single cells simultaneously, allowing for flexibility in scheduling while meeting the specific needs of translational and clinical labs dealing with fragile samples or time constraints that would otherwise impede analysis.

Diagram highlighting the Flex scRNA-seq workflow, featuring stages like cell isolation, barcoding, reverse transcription, library preparation, sequencing, and data analysis. Each step is represented with icons and arrows in a visually organized layout.

Highlights

Preserve biological states and fragile cells by fixing fresh samples at the collection point.
Enhance the efficiency of single-cell workflows through improved sample collection, preparation, and processing steps.
Streamline processes and increase efficiency by batching and multiplexing samples, thereby minimizing experimental variability.
Achieve large-scale and highly sensitive measurements of gene expression.
Unlock new possibilities in single-cell analysis by gaining access to archived samples.

Sample submission requirements

Service	Sample Type	Minimum Amount	Morphology	Debris
Flex scRNAseq	Single cells or Single nuclei	500,000	Intact membrane	Low

Single cells/single nuclei are suspended in frozen media and sent on dry ice
The cells/nuclei will be checked the quality and the core has the rights to determine if the samples are qualified for the experiment
Please contact the core via email at [email protected] or call 713-500-7933 for more information.

Service workflow

Workflow diagram for Flex scRNA-seq showing key steps from sample preparation and barcoding to cDNA synthesis, library construction, sequencing, and analysis. Icons and arrows visually guide the sequential process.

Fixed RNA Profiling with Cell Ranger multi

Cell Ranger v7.0 and later supports analyzing Fixed RNA Profiling (FRP) data with the cellranger multi pipeline (see library compatibility table). FRP data cannot be analyzed with the cellranger count pipeline. | Instructions.