Ultra-low input total RNA sequencing (Ultra-low input total RNA-seq)
This sequencing service is able to provide a solution for those samples which can't increase the amount and improve the quality, such as FFPE samples. SMART® (Switching Mechanism At 5' end of RNA Template) cDNA synthesis technology (Chenchik et al. 1998) and generates Illumina-compatible libraries via PCR amplification, avoiding the need for adapter ligation. The directionality of the template-switching reaction preserves the strand orientation of the original RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA.
Sample submission requirements
| Service | Sample Type | Recommended Amount | Minimum Amount | Minimum Concentration | Quality |
| Ultra-low input total RNA-seq | Total RNA | 10 ng | 5 ng | 1 ng/ul | Must be treated by RNase-free DNase RIN>2.3 DV200>66% |
- RIN stands for RNA integrity number, which is evaluated by measuring the ratio of 28S:18S
- DV200 evaluates the percentage of fragments >200 nucleotides
- This service may not be available for some species
- Concentration must be measured by Qubit
- The core has the rights to determine if the samples are qualified for the experiment
- Please contact the core via email at [email protected] or call 713-500-7933 for more information.
Service workflow
